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. 2023 Sep 20;111(18):2881–2898.e12. doi: 10.1016/j.neuron.2023.06.005

Figure 4.

Figure 4

HTT deletion does not modify global translation

(A) SUnSET assay showing puromycin incorporation revealed by immunoblotting in HTT-deleted MEFs (MEF HTTfl/fl+Cre) versus control MEFs. Unpaired t test, ∗∗∗p value < 0.001.

(B) Representative images and quantification of OPP incorporation in WT RGCs with DMSO or anisomycin injection.

(C) Representative images and quantification of OPP incorporation in intact and 3dpc RGCs.

(D) Representative images and quantification of OPP incorporation in HTTfl/fl+AAV2-Plap and HTTfl/fl+AAV2-Cre intact RGCs. Data are represented as mean ± S.E.M. Unpaired t test; ∗∗∗p value < 0.001; ns: not significant.

(E) Representative images and quantification of OPP incorporation in Ptenfl/fl+AAV2-Cre, Ptenfl/flHTTfl/fl+AAV2-Cre, and Ptenfl/flHTTfl/fl+AAV2-Plap intact RGCs.

(F) Representative images and quantification of OPP incorporation in Ptenfl/fl+AAV2-Cre, Ptenfl/flHTTfl/fl+AAV2-Cre, and Ptenfl/flHTTfl/fl+AAV2-Plap RGCs at 3dpc.

Data are represented as mean ± SEM. One-way ANOVA with Bonferroni multiple comparisons test; ∗∗∗p value < 0.001; ns, not significant.