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. 2023 Sep 20;111(18):2881–2898.e12. doi: 10.1016/j.neuron.2023.06.005

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© 2023 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

HTT controls axon regeneration via the translational regulation of Tox2 mRNA

(A) Representative polysome profile of HTT-deleted (KO) MEF versus control on a 15%–50% sucrose gradient.

(B) Representative Tox2 and Gapdh mRNA distribution in fractions 4–14 of a polysome fractionation, in HTT-deleted (Cre) versus control MEFs.

(C) Tox2 and Gapdh mRNA distribution in monosomal (yellow) and polysomal (purple) fractions. Data are represented as min/max values with line at mean. Paired t test; ^∗p value < 0.05; ns, not significant.

(D) Principle of puro-PLA experiment in vivo.

(E) PLA staining confocal images for puromycin-Tox2 or puromycin-Gapdh in intact RGCs in Pten^fl/fl+AAV2-Cre, Pten^fl/flHTT^fl/fl+AAV2-Cre, and Pten^fl/flHTT^fl/fl+AAV2-Plap.

(F) Quantification of puromycin-Tox2 PLA-positive events in Pten^fl/fl+AAV2-Cre, Pten^fl/flHTT^fl/fl+AAV2-Cre, and Pten^fl/flHTT^fl/fl+AAV2-Plap intact RGCs.

(G) Quantification of puromycin-Gapdh PLA-positive events in Pten^fl/fl+AAV2-Cre, Pten^fl/flHTT^fl/fl+AAV2-Cre, and Pten^fl/flHTT^fl/fl+AAV2-Plap intact RGCs.

(H) PLA staining confocal images for puromycin-Tox2 or puromycin-Gapdh in Pten^fl/fl+AAV2-Cre, Pten^fl/flHTT^fl/fl+AAV2-Cre, and Pten^fl/flHTT^fl/fl+AAV2-Plap RGCs at 3dpc.

(I) Quantification of puromycin-Tox2 PLA-positive events in Pten^fl/fl+AAV2-Cre, Pten^fl/flHTT^fl/fl+AAV2-Cre, and Pten^fl/flHTT^fl/fl+AAV2-Plap RGCs at 3dpc.

(J) Quantification of puromycin-Gapdh PLA-positive events in Pten^fl/fl+AAV2-Cre, Pten^fl/flHTT^fl/fl+AAV2-Cre, and Pten^fl/flHTT^fl/fl+AAV2-Plap RGCs at 3dpc.

Data are represented as mean ± SEM. Kruskal-Wallis with Dunn’s multiple comparisons test; ^∗p value < 0.05, ^∗∗p value < 0.01, and ^∗∗∗p value < 0.001; ns: not significant.