Table 2. Changes in peripheral lymphocyte population by sex over the course of intervention.
Cell type | Men | Women | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Placebo (n = 9) | Probiotics (n = 4) | P for Δ (wk 4–wk 0) | P for Δ (wk 8–wk 0) | P for Δ (wk 12–wk 0) | Placebo (n = 5) | Probiotics (n = 7) | P for Δ (wk 4–wk 0) | P for Δ (wk 8–wk 0) | P for Δ (wk 12–wk 0) | |||||||||||
Δ (wk 4–wk 0) | Δ (wk 8–wk 0) | Δ (wk 12–wk 0) | Δ (wk 4–wk 0) | Δ (wk 8–wk 0) | Δ (wk 12–wk 0) | Δ (wk 4–wk 0) | Δ (wk 8–wk 0) | Δ (wk 12–wk 0) | Δ (wk 4–wk 0) | Δ (wk 8–wk 0) | Δ (wk 12–wk 0) | |||||||||
NK cell | ||||||||||||||||||||
% CD56high NK cell/lymphocytes | −0.20 ± 0.33 | 1.46 ± 0.41 | −0.35 ± 0.26 | −0.54 ± 0.24 | 1.40 ± 0.46 | −0.60 ± 0.37 | 0.50 | 0.94 | 0.59 | 0.02 ± 0.35 | 1.47 ± 0.52 | −0.34 ± 0.26 | 0.11 ± 0.12 | 2.01 ± 0.41 | 0.04 ± 0.17 | 0.77 | 0.44 | 0.23 | ||
% CD56low NK cell/lymphocytes | −2.33 ± 5.77 | −15.32 ± 4.10 | −7.49 ± 4.17 | −4.94 ± 4.48 | −22.04 ± 3.89 | −11.70 ± 3.37 | 0.77 | 0.30 | 0.51 | 1.80 ± 2.83 | −11.23 ± 4.44 | −5.43 ± 2.45 | −2.77 ± 6.16 | −11.10 ± 5.06 | −5.43 ± 5.81 | 0.61 | 0.99 | 0.99 | ||
B cell | ||||||||||||||||||||
% CD19+/total live cells | 0.05 ± 1.51 | 0.53 ± 0.97 | 0.15 ± 1.06 | 1.88 ± 1.15 | 1.90 ± 1.55 | 3.76 ± 1.55 | 0.42 | 0.44 | 0.07 | 2.86 ± 1.51 | −0.81 ± 0.90 | 0.01 ± 1.22 | 2.08 ± 1.53 | 4.01 ± 1.97 | 3.13 ± 1.59 | 0.75 | 0.11 | 0.21 | ||
CD4 T cell | ||||||||||||||||||||
% CD4+/lymphocytes | −4.25 ± 1.92 | 6.41 ± 1.70 | 1.68 ± 1.07 | −6.87 ± 5.26 | 0.94 ± 5.96 | −1.66 ± 5.01 | 0.58 | 0.28 | 0.41 | −4.05 ± 2.58 | 8.78 ± 1.50 | 4.10 ± 2.30 | −7.09 ± 4.35 | −0.73 ± 2.15 | −2.43 ± 2.19 | 0.64 | 0.01 | 0.09 | ||
% Naïve CD4 T cell/CD4+ | −3.69 ± 5.65 | −2.06 ± 7.46 | 3.32 ± 6.65 | −9.92 ± 6.62 | −13.1 ± 5.65 | −5.28 ± 4.34 | 0.51 | 0.33 | 0.39 | 4.58 ± 7.39 | −0.68 ± 6.02 | 5.40 ± 6.45 | −1.08 ± 5.10 | 4.68 ± 4.80 | 9.31 ± 4.88 | 0.53 | 0.51 | 0.64 | ||
% CD4 TCM/CD4+ | 1.57 ± 2.41 | 1.90 ± 2.67 | 3.94 ± 2.72 | 1.40 ± 1.53 | −0.18 ± 3.19 | 1.56 ± 2.39 | 0.96 | 0.64 | 0.57 | 2.45 ± 2.83 | 3.78 ± 2.44 | 5.03 ± 3.91 | 1.87 ± 3.86 | 1.06 ± 3.04 | 2.36 ± 2.84 | 0.92 | 0.56 | 0.59 | ||
% CD4 TEM/CD4+ | 0.06 ± 4.35 | −0.87 ± 4.88 | −7.05 ± 4.46 | 9.46 ± 5.49 | 11.70 ± 4.89 | 3.66 ± 3.35 | 0.21 | 0.12 | 0.13 | −6.08 ± 5.65 | −1.93 ± 4.76 | −8.08 ± 6.17 | −2.61 ± 6.53 | −6.30 ± 6.57 | −11.37 ± 5.39 | 0.73 | 0.66 | 0.71 | ||
% CD4 TEMRA/CD4+ | 2.09 ± 1.04 | 1.09 ± 0.70 | −0.19 ± 0.93 | −0.94 ± 2.12 | 1.56 ± 1.03 | 0.03 ± 0.77 | 0.17 | 0.70 | 0.88 | −0.97 ± 1.29 | −1.16 ± 1.54 | −2.35 ± 1.38 | 1.85 ± 1.20 | 0.59 ± 0.54 | −0.27 ± 0.60 | 0.17 | 0.22 | 0.14 | ||
CD8 T cell | ||||||||||||||||||||
% CD8+/lymphocytes | −2.07 ± 3.26 | −3.07 ± 1.93 | −1.96 ± 2.45 | 4.58 ± 5.41 | 3.78 ± 5.01 | 1.36 ± 4.03 | 0.28 | 0.15 | 0.47 | −7.45 ± 2.26 | −8.90 ± 2.05 | −8.00 ± 2.38 | −3.10 ± 3.68 | −8.31 ± 3.49 | −6.11 ± 3.46 | 0.43 | 0.91 | 0.71 | ||
% Naïve CD8 T cell/CD8+ | −3.69 ± 5.65 | −2.06 ± 7.46 | 3.32 ± 6.65 | −9.92 ± 6.62 | −13.10 ± 5.65 | −5.28 ± 4.34 | 0.51 | 0.33 | 0.39 | 4.58 ± 7.39 | −0.68 ± 6.02 | 5.40 ± 6.45 | −1.08 ± 5.10 | 4.68 ± 4.80 | 9.31 ± 4.88 | 0.53 | 0.51 | 0.64 | ||
% CD8 TCM/CD8+ | 1.57 ± 2.41 | 1.90 ± 2.67 | 3.94 ± 2.72 | 1.40 ± 1.53 | −0.18 ± 3.19 | 1.56 ± 2.39 | 0.96 | 0.64 | 0.57 | 2.45 ± 2.83 | 3.78 ± 2.44 | 5.03 ± 3.91 | 1.87 ± 3.86 | 1.06 ± 3.04 | 2.36 ± 2.84 | 0.92 | 0.56 | 0.59 | ||
% CD8 TEM/CD8+ | 0.06 ± 4.35 | −0.87 ± 4.88 | −7.05 ± 4.46 | 9.46 ± 5.49 | 11.70 ± 4.89 | 3.66 ± 3.35 | 0.21 | 0.12 | 0.13 | −6.08 ± 5.65 | −1.93 ± 4.76 | −8.08 ± 6.17 | −2.61 ± 6.53 | −6.30 ± 6.57 | −11.37 ± 5.39 | 0.73 | 0.66 | 0.71 | ||
% CD8 TEMRA/CD8+ | 2.09 ± 1.04 | 1.09 ± 0.70 | −0.19 ± 0.93 | −0.94 ± 2.12 | 1.56 ± 1.03 | 0.03 ± 0.77 | 0.17 | 0.70 | 0.88 | −0.97 ± 1.29 | −1.16 ± 1.54 | −2.35 ± 1.38 | 1.85 ± 1.20 | 0.59 ± 0.54 | −0.27 ± 0.60 | 0.17 | 0.22 | 0.14 |
Data are presented as the mean ± SEM.
P-value from the t-test for between-group comparisons. The cell surface markers for subsets of T cells were as follows: CD45RA+ CD197+ for naïve, CD45RA- CD197+ for TCM, CDRA45- CD197- for TEM, and CD45RA+ CD197- for TEMRA.
NK cells, natural killer cells; TCM, central memory T cell; TEM, effector memory T cell; TEMRA, effector memory-RA T cell.