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Sodium dodecyl sulfate-PAGE of Borrelia whole-cell lysates (A) and immunoblot analysis with monoclonal antibody H9826 (B). The 12.5% gel was stained with Coomassie brilliant blue, and molecular mass standards (MMS) are shown on the left (in kilodaltons). B. hermsii flagellin, which binds to antibody H9826, was detected by 125I-labeled protein A radiography.
