Fig. 5.

IB-exos restore SIRT1 expression and ERK phosphorylation that are reduced by LPS in HUVECs. A. The mRNA level of SIRT1 was examined by qRT-PCR in HUVECs with different treatments (Control, LPS, LPS + IB-exos). B. The protein level of SIRT1 was examined by immunofluorescence staining in HUVECs with different treatments (Control, LPS, LPS + IB-exos). Scale bar = 50 μm. C. The levels of ERK and p-ERK were examined by Western blotting in HUVECs with different treatments (Control, LPS, LPS + IB-exos). Data was presented as means ± SD from three independent experiments. Statistical analysis was performed using one-way ANOVA. *P < 0.05, **P < 0.01.