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. 2023 Sep 23;4(4):102603. doi: 10.1016/j.xpro.2023.102603

Figure 1.

Figure 1

OMAR photochemical bleaching of embryonic tissue

Photochemical bleaching can be performed using intense cold-white LED lights in two ways.

(A) Using LED spotlight light with gooseneck glass fiber transmission (VWR VisiLight LED8, goosenecks provide a 1600 lumen spotlight).

(B) Alternatively, use daylight lamp pads for Light therapy (homogenous LED lighting with 20000 lumen). The cases in panels A and B are homemade to provide the necessary spacing, eye protection from light reflection, and optimal heat management. Details for 3D printing the cases are available on request.

(C) Bubble formation during the 2 × 45 min OMAR photochemical bleaching.

(D) Side-by-side comparison of autofluorescence in four confocal channels (488, 514, 594, and 647 nm) showing the contralateral forelimb buds embryo without (left) and with OMAR photochemical bleaching (right), respectively. Scale bar: 200 μm.