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. 2023 Sep 23;4(4):102603. doi: 10.1016/j.xpro.2023.102603

Blocking Solution

Reagent Final concentration Amount
10% Triton X-100 0.4% 2 mL
10% BSA 1% 5 mL
10% Tween 20 0.1% 0.5 mL
Donkey serum 10% 5 mL
10× PBS (pH 7.4) 1× 5 mL
ddH2O N/A 32.5 mL
Total N/A 50 mL