Figure 6.

Constant optogenetic stimulation of AgRP neurons in the training context.Agrp-ires-cre and AgRP-WT littermates received bilateral injections of AAV9-hsyn-FLEX-soCoChR-GFP into the arcuate nucleus with an indwelling fibre optic cannula implanted above the injection site, creating AgRP^WT (n = 5) and AgRP^SocoChR (n = 7) groups, respectively. (A) Schematic created with BioRender.com outlining conditions of optogenetic stimulation given on all training days for the context-induced feeding experiment. Fed mice received 10 min of pre-stimulation in the home cage, followed by 30 min stimulation for the entire training period in context and an additional 10-min period with stimulation once returned home cages. No food was available during training sessions. (B) Schematic image highlighting the onset and offset of AgRP photostimulation in the absence of food during training relative to the time spent in context A. (C) Context-induced feeding Test. Froot Loop intake in 20-min testing sessions in contexts A and B. (D) Home cage feeding with AgRP photostimulation (student's t-test). Values are presented as mean ± SEM, ∗∗p < 0.01. All specific statistical information is reported in Supplemental Table 1.