Fig. 1.

Isolation of Idit RNAi as a genetic modifier of the Atg1-Atg13-gain-of-function eye phenotype. (A) Schematic representation of the genetic screening for genetic modifiers of Atg1 and Atg13 in Drosophila. (B) Eyes from the flies expressing indicated transgenes were imaged using light dissection microscopy. (C) Eyes from the flies expressing indicated transgenes were subjected to immunoblotting examining indicated proteins. Open arrow indicates phosphorylation-induced electromobility retardation, which shifts the Atg13 band from its unphosphorylated location (closed arrow). (D) Eyes from the flies expressing indicated transgenes.