Fig. 3.
Generation and characterization of Idit gene-trap mutants. (A) Schematic genomic organization of the Idit (CG33143) locus and Idit mutants. Triangles indicate transposon insertions. Open boxes: untranslated exons of Idit or other genes; closed boxes: protein-coding exons of Idit; size bar: relative length of 1 kb genomic span. (B) Schematics of how we converted original MiMIC allele (IditMI03535) into MiMIC gene-trap allele (IditB6). (C–E) Strong IditB6>GFP expression detected in adult skeletal muscle (C), heart (D) and brain (E) tissues. (F–H) Immunoblot analysis of indicated proteins in fly tissues of indicated genotypes. (I–L) Lifespan analysis of flies with indicated genotype and treatment (n ≥ 70 for all groups presented). P < 0.001 between y w and IditB6 (I and SY5 in J), IditB6 and IditB6 Rescue (K), and control and Idit-overexpressing groups (L), in log-rank test.