Fig. 5.

Induction of Idit expression is sufficient to improve endurance. (A and B) Idit mRNA expression was measured by quantitative RT-PCR. Measurements took place in outcrossed Mef2-Gal4 flies to better match the genetic background of overexpression experiments in B (n = 3; each set includes 10 flies). For A, exercised (EX) or control-treated (UN) flies were examined at 25 d of age. For B, unexercised flies were examined at 5 d of age. (C) Baseline endurance was measured at 5 d of age (n = 8; unpaired t-test). MHC-GS-Gal4 was used to control Idit expression. ON indicates groups fed 100 µm mifepristone and OFF indicates groups fed vehicle solution. (D) Endurance of exercised (EX) or control (UN) flies was measured at 25 d of age (n = 8; two-way ANOVA with Tukey multiple comparison; genotype by interaction effect P = 0.27, genotype effect P = 0.002, exercise effect P = 0.0007). (E) Time to recovery following a 2-h chill coma (n = 24; unpaired t-test). (F) Immunoblot analysis of indicated proteins in fly tissues of indicated genotypes. Open and closed arrows indicate unprocessed (Atg8a-I) and processed (Atg8a-II) Atg8a proteins, respectively. (G) Lysotracker analysis of indirect flight muscle from flies of indicated genotypes. Brightfield images corresponding fluorescence image was shown below. Boxed areas are magnified in Right. Error bars represent SD. Asterisks indicate significance from one-way ANOVA, two-way ANOVA, or t test as indicated above; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.