Fig. 1.

T cell–mediated cytotoxicity induces FAO elevation in cancer cells. B16 melanoma cells were cocultured with Pmel cells for 6 h. The FAO rate in cancer cells was determined by measuring the oxygen consumption rate (OCR) using Seahorse assays following extensive wash to remove T cells (A). The expression of CPT1 enzymes in B16 melanoma (B) or RM1-OVA prostate cancer cells (C) was examined by immunoblotting. Densitometry analysis of immunoblot replicates for CPT1A expression is shown. Data are representative of at least three independent experiments. *P < 0.05.