Skip to main content
. 2023 Sep 18;120(39):e2220062120. doi: 10.1073/pnas.2220062120

Fig. 4.

Fig. 4.

Reductions in cytoplasmic macromolecular crowding and nuclear translocation of transcription factors after recovery from compression. (A) Immunofluorescent images of uncompressed and recovered B16F0 cells. (B) The normalized cytoplasmic mean fluorescence intensity (MFI) of β-catenin is shown in the scatter plots (n ≥ 18 wells for each condition, from two independent experiments). (C) The percentage of translocated β-catenin inside the nucleus is shown in the scatter plots (n ≥ 12 wells for each condition, from two independent experiments). (D) Immunofluorescent images of uncompressed and recovered B16F0 cells. (E) The normalized cytoplasmic MFI of YAP/TAZ is shown in the scatter plots (n ≥ 18 wells for each condition, from two independent experiments). (F) The percentage of translocated YAP/TAZ inside the nucleus is shown in the scatter plots (n ≥ 12 wells for each condition, from two independent experiments). All error bars are mean with SD. Details of statistical analyses used can be found in Materials and Methods—Statistical Analysis.