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. 2023 Sep 21;120(39):e2300348120. doi: 10.1073/pnas.2300348120

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Copyright © 2023 the Author(s). Published by PNAS.

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Fig. 2. — CART detection in C bouton synapses and in a subset of spinal motor neurons. (A, Ai and Aii) Immunohistochemistry in P25 mice with antibodies against ChAT (red) and CART (green) reveals the presence of CART puncta in the motor neuron area, specifically on motor neuron membranes. CART immunoreactivity is also observed in a few motor neuron somata (white arrowhead). (Scale bar, 20 μm.) (B, Bi and Bii) Colocalization of ChAT (red) and CART (green) in the cholinergic C bouton synapses on motor neurons of P8 mice. (C, Ci and Cii) C bouton synapses of P25 mice also contain CART. (Scale bars, 2 μm (B and C).]