Fig. 2.

BRWD3 and KDM5 are in the same complex and bind the same genomic regions. (A) Workflow of BRWD3-HA IPs from Drosophila embryos followed by mass spectrometry. (B) Volcano plot of the BRWD3-IP-TMT-MS results. Blue dots represent hits significantly enriched in BRWD3-HA-IP compared to the negative control. The red dots represent the Cul4-DDB1-BRWD3 E3 ubiquitin ligase complex. (C) Representative view showing the similarity of BRWD3 and KDM5 ChIP-seq tracks (Left). Venn diagram quantifying the overlap between BRWD3 and KDM5 binding sites on the genome, P < 0.0001 (Right).