Fig. 7.
R406/R788 promote the cancer-killing activity of TRAIL. (A) E0771 cells treated with TRAIL, with or without three different concentrations of R406, for 24 h. Representative dose–response curves, tabular listing of IC50 values for trail with or without increasing concentrations of R406. (B) E0771 tumor cells were treated with 3 μM R406 for 30 min and then 10 ng/mL mTRAIL was added for various time points. The cell lysates were analyzed by western blotting. (C) Schematic diagram of the experimental schedule. E0771 cells were engineered to express. ZsGreen luciferase (ZsGreen-LUC-expressing E0771). After SC injection 11 and 15 d, C57BL/6 (6 to 8 wk old, female), were injected with D-Luciferin (150 mg/kg) in situ, respectively. Progression of breast tumor burden and treatment effects in mice were monitored in vivo and ex vivo by bioluminescent imaging (BLI). (D) ZsGreen-LUC-expressing E0771 tumor cells were implanted in 6-wk-old C57BL/6 WT female mice. After 16 d, collected the tumor tissues of female mice. (E) ZsGreen-LUC-expressing E0771 tumor parameters in C57BL/6 mice during indicated treatment. Mean ± SEM of n = 8. ***P < 0.001.