TABLE 1.

Quantification of HGE agent organisms by competitive PCR, percentage of morulae in granulocytes, and infectivity of blood from an SCID mouse infected with the HGE agent

Blood	Dilution	No. of bacteria/mla	% Morulae (mean ± SD)b	Infectivityc
Infected	Neat	1.2 × 108	9.0 ± 0.0	4/4
	1:10	1.2 × 107	11.4 ± 0.6	4/4
	1:100	9.6 × 105	5.6 ± 0.4	4/4
	1:1,000	1.2 × 105	0	2/4
Control	Neat	0	0	0/4

^aHGE agent bacterial numbers were estimated on the basis of 1.9 × 10⁵ bacteria/50 fg of target DNA. 

^bPercentage of granulocytes with morulae among 200 granulocytes examined per mouse. 

^cNumber of C3H mice with PCR-positive blood/number of mice inoculated with 0.1 ml of each dilution of blood.