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. Author manuscript; available in PMC: 2024 Oct 1.
Published in final edited form as: Hepatology. 2023 Apr 11;78(4):1209–1222. doi: 10.1097/HEP.0000000000000401

Figure 2. Inducing senescence in hepatocytes up-regulates hepatocyte expression of THBD-PAR1 and inhibits liver regeneration.

Figure 2.

AAV8-TBG-eGFP-P16 (P16) or its control vector, AAV8-TBG-eGFP (GFP), were administered to C57BL/6 wild-type mice via tail vein injection. (A) Representative micrographs of GFP immunohistochemistry and P16-RT-PCR-ISH (Reverse Transcript polymerase chain reaction-in situ hybridization), (B) Hematoxylin & Eosin staining in liver tissue. (C) Serum biochemical parameters (ALT, Albumin and Bilirubin). (D) SA-β-Gal staining and THBD- and PAR1- immunostaining of liver sections from GFP or P16 over-expressing mice. One week after the AAV injection, mice underwent 70% PHx and were sacrificed 48h or 72h later. (E) Liver to body weight ratios from GFP and P16 over-expressing mice after PHx. (F) Markers of hepatocyte proliferation after PHx in liver sections from GFP and P16 over-expressing mice. Results shown as MEAN +/− SEM (n=3 mice/group/time, *p<0.05). Micrographs 100X magnification.