Figure 5:

Depletion of emc9 or emc10 in Xenopus affects WNT signaling via depletion of transmembrane protein levels. (A) Immunoblotting for Fzd7 showed a decrease in Fzd7 in pooled (n = 30 per stage per condition) emc9 depleted and emc10 depleted tadpoles as compared with control tadpoles at stage 45. (B) Immunoblotting for β-catenin showed a marked decrease in nuclear β-catenin in pooled (n = 30 per stage per condition) emc9 depleted and emc10 depleted tadpoles as compared with control tadpoles at stage 45.