Key resources table

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal Anti-AMID	Santa Cruz Biotechnology	Cat# sc-377120
Mouse monoclonal Anti-β-actin	Santa Cruz Biotechnology	Cat# sc-47778
Rabbit polyclonal Anti-GPX4	Abcam	Cat# ab125066
Rabbit polyclonal Anti-ACSL4	Sigma-Aldrich	Cat# SAB2701949
Rabbit polyclonal Anti-AIFM2	LS Bio	Cat# LS-C382008-50
Mouse monoclonal Anti-GAPDH	EMD Millipore Corp	Cat# MAB374
Bacterial and virus strains
LOBSTR-BL21 (DE3) competent cells	Kerafast	Cat# EC1002
Stbl3 competent cells	Macrolab at UC Berkeley	N/A
NEB 5DH-α	NEB	Cat# C278H
Biological samples
Mouse microsomes	Xenotech	Cat# M1000
Chemicals, peptides, and recombinant proteins
RSL3	Cayman Chemical	Cat# 19288
Fer-1	Cayman Chemical	Cat# 17729
Idebenone	Cayman Chemical	Cat# 15475
Deferoxamine mesylate (DFO)	Cayman Chemical	Cat# 14595
ML162	Cayman Chemical	Cat# 20455
ZVAD(OMe)-FMK	Cayman Chemical	Cat# 14463
Necrostatin-1	Cayman Chemical	Cat# 11658
Dihydroartemisin (DHA)	Selleck Chemical	Cat# S2290
Puromycin	Thermo Fisher Scientific	Cat# A1113802
SYTOX Green Dead Cell Stain	Thermo Fisher Scientific	Cat# S34860
Polybrene	Sigma-Aldrich	Cat# TR-1003
Coenzyme Q1	Sigma-Aldrich	Cat# C7956
CoQ-Coumarin	Cayman Chemical	Cat# 29554
Resazurin	Thermo Fisher Scientific	Cat# R12204
Bodipy C11	Thermo Fisher Scientific	Cat# D3861
NADH	Millipore Sigma	Cat# 481913
Small Molecule Library	ChemDiv	N/A
FDA-Approved Drug Library	TargetMol	Cat# L4200
Bioactive Compound Library	TargetMol	Cat# L4000
Hygromycin	Thermo Fisher Scientific	Cat# J67371.XF
Bleomycin	Thermo Fisher Scientific	Cat# J60727.MCR
Recombinant FSP1 (Human)	This Study	N/A
Recombinant FSP1 (Mouse)	This Study	N/A
Critical commercial assays
CellTiter Glo Luminescent Cell Viability Assay	Promega	Cat# G7572
Pierce BCA Protein Assay Kit	Thermo Fisher Scientific	Cat# PI23227
PCR Cloning System with Gateway® Technology	Thermo Fisher Scientific	Cat# 12535-029
Experimental models: Cell lines
Human: NCI-H460	ATCC	HTB-177
Human: A375	UC Berkeley Cell Culture Facility	N/A
Human: HCC1143	UC Berkeley Cell Culture Facility	N/A
Human: Huh7	UC Berkeley Cell Culture Facility	N/A
Human: NCI-H460C	This Study	N/A
Human: A549N	Forcina et al. 2022	N/A
Human: HCC1143C	Li et al. 2022	N/A
Human: NCI-H460C GPX4KO	This Study	N/A
Human: NCI-H460C FSP1KO	This Study	N/A
Human: HT-1080N	Forcina et al. 2022	N/A
Human: U-2OSN	Forcina et al. 2022	N/A
Human: T98GN	Forcina et al. 2022	N/A
Human: Huh7C	This Study	N/A
Human: 501-MEL	Kind gift from Imanol Arozarena Martinicorena at Navarrabiomed	N/A
Human: SKMEL28	Kind gift from Imanol Arozarena Martinicorena at Navarrabiomed	N/A
Human: NCI-H460C FSP1KO mFSP1 OE	This Study	N/A
Experimental models: Organisms/strains
Balb/C mice	Envigo	N/A
Oligonucleotides
See Table S1
Recombinant DNA
pET-His6-TEV vector	Addgene	Cat# 29653
hFSP1-pET-His6-TEV vector	Bersuker et al. 2019	N/A
mFSP1-pET-His6-TEV vector	This paper	N/A
NQO1-pET-His6-TEV vector	This paper	N/A
GenEZ ORF Clone: Aifm2_OMu13438D_pcDNA3.1+/C-(K)-DYK	GenScript	Cat# SC1200
CSII-prEF1a-mCherry-3xNLS	Addgene	Cat# 125262
mCherry pMCB320	Addgene	Cat# 89359
pENTR1A-GFP-N2	Addgene	Cat# 19364
mFSP1-pENTR1A	This paper	N/A
pLenti CMV mFSP1 Hygro	This paper	N/A
Software and algorithms
Prism 9	GraphPad Software	N/A
R Studio	Posit Software, PBC	https://posit.co/download/rstudio-desktop/
Microsoft Excel	Microsoft Corporation	N/A
FlowJo	Becton, Dickinson & Company	N/A
SynergyFinder R package	Bioconductor	https://www.bioconductor.org/packages/release/bioc/html/synergyfinder.html
Oligonucleotides
His-pET-hNQO1 I-PIPE primer Forward 5’- CTG TAC TTC CAA TCC AAT ATT GTC GGC AGA AGA GCA CTG ATC −3’	This paper	N/A
His-pET-hNQO1 I-PIPE primer Reverse 5’- TCG GAT CCG TTA TCC ACT TCC TCA TTT TCT AGC TTT GAT CTG GTT GTC −3’	This paper	N/A
His-pET-hNQO1 V-PIPE primer Forward 5’ CAG ATC AAA GCT AGA AAA TGA GGA AGT GGA TAA CGG ATC CGA −3’	This paper	N/A
His-pET-hNQO1 V-PIPE primer Reverse 5’- CAG TGC TCT TCT GCC GAC AAT ATT GGA TTG GAA GTA CAG −3’	This paper	N/A
His-pET-mFSP1 I-PIPE primer Forward 5’- CTG TAC TTC CAA TCC AAT ATT GGG TCC CAG GTC TCG GTG GAT AC −3’	This paper	N/A
His-pET-mFSP1 I-PIPE primer Reverse 5’ TCG GAT CCG TTA TCC ACT TCC TTA CGG TGG AGA CTG CCG CAT −3’	This paper	N/A
His-pET-mFSP1 V-PIPE primer Forward 5’ ATG CGG CAG TCT CCA CCG TAA GGA AGT GGA TAA CGG ATC CGA ATT C −3’	This paper	N/A
His-pET-mFSP1 V-PIPE primer Reverse 5’- GT ATC CAC CGA GAC CTG GGA CCC AAT ATT GGA TTG GAA GTA CAG G −3’	This paper	N/A
mFSP1 I-PIPE primer Forward 5’- AAG GAA CCA ATT CAG TCG ACT GGA TGC CAC CAT GGG GTC C −3’	This paper	N/A
mFSP1 I-PIPE primer Reverse 5’- GTC TAG ATA TCT CGA GTG CGG CCG CTT CAC GGT GGA GAC T −3’	This paper	N/A
mFSP1 V-PIPE primer Forward 5’- AGT CTC CAC CGT GAA GCG GCC GCA CTC GAG ATA TCT AGA C −3’	This paper	N/A
mFSP1 V-PIPE primer Reverse 5’- GGA CCC CAT GGT GGC ATC CAG TCG ACT GAA TTG GTT CCT T −3’	This paper	N/A