TABLE 3.
Sensitivities of different pools of HCMV pp65-specific monoclonal antibodies with formalin plus NP-40 as fixative (prior to storage at −80°C) and IFA as immunostaining technique
| Monoclonal antibody pool (no. of monoclonal antibodies)a | Median no. of pp65-positive PMN (range) | P valueb | Median no. (%) of pp65-positive PMN with degree of staining
|
P valuec | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Weak | Moderate | Strong | ||||||||
| PV pool (3) | 205.5 (160–240) | 51.5 (25) | 130.5 (64) | 23.5 (11) | ||||||
| Argene (2) | 169 (140–210) | 0.0041 | 64 (38) | 87.5 (52) | 17.5 (10) | 0.022 | ||||
| 0.0047 | 0.046 | |||||||||
| Biotest (2) | 138 (117–153) | <0.00001 | NSd | 58.5 (43) | 75 (54) | 4.5 (3) | 0.0003 | NS | ||
| NS | NS | |||||||||
| Chemicon (2) | 152.5 (110–179) | 0.0008 | 57.5 (38) | 83 (54) | 12 (8) | 0.034 | ||||
a
For each pool, data for 10 replicates are shown.
b
t test.
c
Pearson chi-square.
d
NS, not significant.