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. Author manuscript; available in PMC: 2024 Aug 1.
Published in final edited form as: Small. 2023 May 10;19(34):e2301801. doi: 10.1002/smll.202301801

Figure 2. Immune responses induced by intramuscular immunization of different protein nanoparticle vaccines.

Figure 2.

Sera were collected and analyzed three weeks post-boost immunization. (A) M2e, NA1, and NA2 specific IgG levels after boost. (B), (C) and (D) NA2, NA1, and M2e specific IgG isotypes after boost. Groups of mice were euthanized at week four post the boosting immunization. The spleens and bone marrow were collected and processed into single-cell suspensions for analysis. (E) and (F) NA-specific IL-4 and IFN-γ-secreting cells in spleens. (G), (H) and (I) Aichi-, PR8- and M2e- specific antibody-secreting cells in the bone marrow. The histograms were presented as mean ± SEM. Statistical significance was analyzed by F-test and two-tailed t-test. *p < 0.05; **p < 0.01; ***p < 0.001. The results show one experiment with two replicates for each sample. Mice groups were n = 5.