Skip to main content
. Author manuscript; available in PMC: 2024 Aug 1.
Published in final edited form as: J Mol Cell Cardiol. 2023 May 24;181:1–14. doi: 10.1016/j.yjmcc.2023.05.004

Figure 8. YAP promotes the transcription of inflammasome related genes.

Figure 8.

(A and F) Schemas representing the mouse Nlrp3 and Il-1b gene proximal promoters. Transcription start sites (TSS) and conserved TEAD motifs (grey boxes) are shown. (B-C) Chromatin immunoprecipitation (ChIP) assays were carried out using RAW264.7 cells transfected with GFP or YAP expression vectors and anti-YAP antibody. Separate primer sets (P1 or P2) were used to detect distinct regions of the Nlrp3 promoter. (D-E) HEK293 cells were transfected with luciferase reporter constructs cloned from 2 separate regions of the Nlrp3 promoter (L1 or L2) in combination with GFP or YAP plasmids, in the presence or absence of verteporfin (VP; 1 μM). (G-H) ChIP assays performed in RAW264.7 cells using 2 different primer sets (P1 or P2) to detect distinct regions of the Il-1b promoter. (I-J) Luciferase assays conducted in HEK293 cells using reporter constructs generated from 2 separate regions of the Il-lb promoter (L1 or L2). N = 3-4 experimental replicates. All data are presented as mean ± SEM. P values were determined by ordinary one-way ANOVA with multiple comparison. *P<0.05, **P<0.01, ***P<0.001.