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. Author manuscript; available in PMC: 2023 Sep 27.
Published in final edited form as: Curr Eye Res. 2023 May 7;48(8):736–749. doi: 10.1080/02713683.2023.2206067

Fig. 3. Effect of simvastatin on pMLC levels in HTM cells.

Fig. 3.

(A) Representative fluorescence micrographs of p-MLC in HTM cells cultured atop ECM hydrogels subjected to vehicle control, dexamethasone (D; 100 nM), simvastatin (S; 10 μM), dexamethasone + simvastatin, and dexamethasone + simvastatin + mevalonate-5-phosphate (M; 500 μM) at 3 d. Scale bar, 20 μm. (B) Analysis of p-MLC fluorescence intensity (N = 29-33 images from 3 HTM cell strains with 3 experimental replicates per cell strain). Symbols with different colors represent different cell strains; dotted line indicates control baseline. The bars and error bars indicate Mean ± SD. Significance was determined by two-way ANOVA using multiple comparisons test; shared significance indicator letters = non-significant difference (p>0.05), distinct letters = significant difference (p<0.05).