FIG 1.

Drug-reactive CD8⁺ T-cell responses are enriched in FLX-primed Tg mice. CD8⁺ T cells were isolated from spleens of drug-naive HLA-B*57:01⁺ (Tg) and WT control animals (A and B) or FLX-treated Tg mice (B) and cultured with irradiated autologous feeders at a 1:2 ratio in the presence or absence of FLX or ABC (125 μg/mL of FLX and 10 μg/mL of ABC in (B)). IFN-γ and GZMB levels were measured at different time points by ELISA. (A) Data shown are from 1 representative of 2 independent experiments. (B) IFN-γ values are presented as means ± SEMs of results from 3 animals per group. (C) Cell morphology changes observed by optical microscopy (20×) at day 4 of culture of CD8⁺ splenocytes from a representative FLX-primed animal, in the absence or presence of 125 μg/mL of FLX.