KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| APC, anti-NHP CD45, clone D058-1283 | BD Biosciences | Cat #561290; RRID: AB_10613814 |
| PE-Cy7, mouse anti-NHP CD45, clone D058-1283 | BD Biosciences | Cat #563861; RRID: AB_10612014 |
| FITC, mouse anti-human CD20, clone 2H7 | BD Biosciences | Cat #555622; RRID: AB_395988 |
| Pacific Blue, mouse anti-human CD3, clone SP34-2 | BD Biosciences | Cat #558124, RRID: AB_397044 |
| ECD, mouse anti-human CD14, clone RMO52 | Beckman Coulter | Cat #IM2707U |
| PerCP-Cy5.5, mouse anti-human CD4, clone L200 | BD Biosciences | Cat #552838, RRID: AB_394488 |
| APC-H7, mouse anti-human CD8alpha, clone SK1 | BD Biosciences | Cat #561423, RRID: AB_10682894 |
| FITC, mouse anti-human CD16, clone 3G8 | BD Biosciences | Cat #555406 |
| Pacific Blue, mouse anti-human CD8, clone DK25 | Agilent | Cat #PB98401-1 |
| Alexa Fluor 700, mouse anti-human CD3, clone SP34-2 | BD Biosciences | Cat #557917, RRID: AB_396938 |
| PerCP-Cy5.5, mouse anti-human CD8alpha, clone SK1 | BD Biosciences | Cat #341051, RRID: AB_400209 |
| PE, mouse anti-human CD8beta, clone 2ST8.5H7 | Beckman Coulter | Cat #IM2217U |
| APC-H7, mouse anti-human CD20, clone 2H7 | BD Biosciences | Cat #560853, RRID: AB_10561681 |
| PE-Cy7, mouse anti-human CD4, clone OKT4 | Biolegend | Cat #317414, RRID: AB_571959 |
| PE, mouse anti-human CD28, clone 28.2 | BD Biosciences | Cat #556622, RRID: AB_396494 |
| PE-Cy7, mouse anti-human CD95, clone DX2 | BD Biosciences | Cat #561636, RRID: AB_10896323 |
| Pacific Blue, mouse anti-human CD8alpha, clone RPA-T8 | BD Biosciences | Cat #558207, RRID: AB_397058 |
| FITC, mouse anti-human CD95, clone DX2 | BD Biosciences | Cat #556640, RRID: AB_396506 |
| PerCP-Cy5.5, mouse anti-human CD279/PD-1, clone EH12.2H7 | Biolegend | Cat #329914, RRID: AB_1595461 |
| Alexa Fluor 700, mouse anti-human CD4, clone L200 | BD Biosciences | Cat #560836, RRID: AB_10563410 |
| PE-Cy7, mouse anti-human CD185/CXCR5, clone MU5UBEE | eBioscience | Cat #25-9185-42, RRID: AB_2573540 |
| PE, mouse anti-human CD34, clone 561 | Biolegend | Cat #343606, RRID: AB_1732008 |
| Bacterial and Virus Strains | ||
| SIVmac239 | Lab-generated | Stock 20082 |
| SHIV-AD8-EOM | Laboratory of Dr. Brandon Keele | Stock SP2105A_2019 |
| Experimental Models: Organisms/Strains | ||
| Mauritian-origin Macaca fascicularis | Accredited vendors: Worldwide Primates, Inc., PrimeGen, LLC, or Charles River Primates, Inc. | N/A |
| Oligonucleotides | ||
| SIV/SHIV qPCR detection primers and probe: SGAG21 forward primer 5'-GTCTGCGTCATPTGGTGCATTC-3', SGAG22 reverse primer 5'-CACTAGKTGTCTCTGCACTATPTGTTTTG-3', pSGAG23 probe 5′-6-carboxyfluorescein [FAM]-CTTCPTCAGTKTGTTTCACTTTCTCTTCTGCG-black hole quencher [BHQ1]-3′ | Integrated DNA Technologies | N/A |
| MHC typing primers: MHC I 5′-GCTACGTGGACGACACG and 5′-TCGCTCTGGTTGTAGTAGC, MHC II 5′-CGCTTCGACAGCGAC and 5′-ACTCGCCGCTGCA | Integrated DNA Technologies, see reference 30 | N/A |
| Donor chimerism SNP-specific primers, see reference 30 Supplementary Table 1 for sequences | Integrated DNA Technologies | N/A |
| Software and Algorithms | ||
| FlowJo v10.8.1 | BD Biosciences | N/A |
| Prism v9.4.0 | GraphPad Software | N/A |
| Halo v3.2.1851.393 (FISH module v3.0.4) | Indica Labs | N/A |
| SAS v9.4 | SAS Institute | N/A |
| Geneious Prime 2022.1.1 | Biomatters Ltd | N/A |