Figure 1. Analysis of the age-related hematological phenotypes induced by Tet2 Mut and KO mouse bone marrow cells.
A. Schematic of transplantation of bone marrow from Tet2 Mut and KO mice at the indicated ages. 4.0 × 105 bone marrow mononuclear cells (BM-MNCs) from 3-, 6-, 9- and 12- month-old Tet2 Mut or KO primary mice with no sign of hematological disorders, or age-matched wild-type mice (Ly5.2) were transplanted into irradiated recipient mice (Ly5.1) with 4.0 × 105 competitor BM-MNCs (Ly5.1).
B. Kaplan-Meier survival curve of transplanted mice (n = 10 per each group). Log-rank test was used to assess statistical significance (p-value).
C. Median survival of transplanted mice. Note: control wild-type cohort survived beyond 240 days.
D. Disease spectrum of the recipient mice from entire cohort (3, 6, 9 and 12 months old, n = 40). Representative flow data and percentages of the indicated fractions of the donor-derived cells in the peripheral blood of recipient mice transplanted with Tet2 Mut and KO donor bone marrow cells which developed hematological disorders are shown in lower panels. Representative gating strategy of donor-derived cells (green) is also shown in bottom left.
E. Disease spectrum of each cohort (n = 10 per each group).
In all panels, error bars indicate SD unless otherwise specified. n.c. stands for no significant change. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.