Fig. 10.

Fluorescence images of A459 cells subjected to various pre-treatment conditions, such as lactate, pyruvate, or a mixture of the two in cell medium, for an hour before being incubated with 5 μM of probe A or B in cell medium for a further hour. Images of the fluorescent emission from these cells were then collected at 488 nm excitation and their signals were analyzed at the corresponding wavelength ranges (575 nm to 675 nm for probe A, and 550 nm to 650 nm for probe B). The resulting images, featuring probe A on the left and probe B on the right, are shown. The scale bars on the images represent 50 μm.