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. 2023 Sep 25;15(2):2257269. doi: 10.1080/19490976.2023.2257269

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© 2023 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 1. — IEC-derived GPR65 preserves intestinal antimicrobial activity. (a-c) qPCR analysis of the indicated genes in IECs from the large bowels of Gpr65^fl/fl and Gpr65^ΔIEC mice. (d-f) qPCR analysis of the indicated genes in IECs from the small bowels of Gpr65^fl/fl and Gpr65^ΔIEC mice. (g) representative immunofluorescence staining of REG3γ in both large and small bowels from Gpr65^fl/fl and Gpr65^ΔIEC mice. Scale bars, 100 μm. (h, i) immunoblotting analysis and quantification of REG3γ in the IECs from the large bowels of Gpr65^fl/fl and Gpr65^ΔIEC mice. (j, k) information for cellular localization and relative expression level of GPR65 retrieved from publicly available data. *p < 0.05, **p < 0.01. Data are representative of three independent experiments.