Figure 3.

Mice deficient for epithelial GPR65 are prone to DSS-induced colitis. Gpr65^ΔIEC mice and Gpr65^fl/fl littermates (n = 6 in each group) were administered 2% DSS in drinking water for 7 days, followed by 3 days of regular water. (a) the body weight changes of Gpr65^fl/fl and Gpr65^ΔIEC mice during DSS-induced colitis. (b) gross morphology of colons on day 10 when mice were sacrificed. (c) colon length on day 10 of the colitis model. (d) representative sections of the distal colon tissues after H&E staining. Scale bars, 100 μm. (e) pathological scores of the colon sections were calculated as indicated. (f) FITC-dextran (μg/mL) in sera. (g-k) relative Il6, Tnfa, Cxcl1, Lcn2 and Il22 mRNA expression in the distal colon tissues of indicated groups of mice. (l-n) relative Reg3g, Reg3b and Nos2 mRNA expression in the colonic IECs of indicated groups of mice. (o-p) immunoblotting analysis and quantification of REG3γ in the colonic IECs. *p < 0.05, **p < 0.01. Data are representative of three independent experiments.