Figure 4. Genome-scale CRISPR-Cas9 screen identifies CMTM6 as a positive regulator of CD58.

(A) Experimental design of whole genome-wide CRISPR-Cas9 KO screen to identify positive regulators of CD58. Screen was performed independently twice, with two technical replicates each.

(B) Distribution of CRISPR-Cas9 target gene enrichment within the CD58^lo population compared to the CD58^mi population from an example replicate, showing log2 fold change of gene enrichment and P-value for enrichment calculated from a negative binomial model as determined by the Model-based Analysis of Genome-wide CRISPR-Cas9 Knockout (MAGeCK) algorithm⁴⁸.

(C) Genes identified as positive regulators of CD58 (criterion of FDR<0.25 across all four replicates), ranked by log2 fold change enrichment of targeting sgRNAs in CD58^lo versus control CD58^mi population across replicates.

Data represent mean ± SD.

See also Figure S5 and Table S2 and S3.