Fig. 4.

CVID/GLILD patients exhibit increased frequency of exhausted CD57⁺ CD8⁺ T cells. Percentages of CD8 T cell populations derived from manual gating of mass cytometry data for naïve CD8⁺ (a), central memory CD8⁺ (b), effector memory CD8 ⁺ (c), TEMRA CD8⁺ (d), ICOS⁺CD8⁺ (e), PD-1⁺CD8⁺ (f), HLADR⁺CD8⁺ (g), CD57 ⁺CD8⁺ (h), naïve CD57⁺CD8⁺ (i), central memory CD57⁺CD8⁺ (j), effector memory CD57⁺CD8⁺ (k), and TEMRA CD57⁺CD8⁺ (l). Unsupervised PhenoGraph clustering of CD8⁺ single cell events (m), cluster map surface marker expression (n), and cluster frequencies corresponding to CD57⁺CD8⁺ TEMRA (cluster 12 and cluster 22), HLADR⁺CD57⁺CD8⁺ TEMRA (cluster 15), and CD57⁺CD8⁺ central memory (cluster 20) (o). Error bars represent median and 95% CI. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Not significant unless stated by asterisk in figure. Kruskal–Wallis accounting for multiple comparisons was used for statistical significance