Figure 1. In vitro efficacy of JAK inhibitors.

A) Table of primary and secondary targets of JAK inhibitors.

B) Cell viability assay of a cell line panel treated with JAK inhibitors. Cells were treated for 72 hours at the indicated drug doses. N= 6 replicates per condition.

C) Apoptosis assay of HEL and UKE-1 cells treated with JAK inhibitors at the indicated doses and duration. N = 3 replicates per condition.

D) Cell cycle assay of HEL and UKE-1 cells treated with JAK inhibitors at the indicated doses and duration. N = 3 replicates per condition.

E) CD34+ colony assay of eight MPN (colored dots) and three NBM (black dots) patient samples. Cells were seeded in MethoCult H4034 in the presence of 1 μM JAK inhibitors and grown for 14 days. Cells were plated in duplicate and numerated.

F) Relative potency ranking of JAK inhibitors from E).