Fig. 4.

MALDI-MS spectrums of Nα methylated peptides. A. o-NBS-protected MeSET-10 peptide. A test cleavage was performed after dimethyl sulfate methylation to determine whether the reaction was completed. A methylation reaction should be performed again if starting material was observed. B. Monomethylated SET10 peptide. C. Dimethylated SET10 peptide. D. Trimethylated SET10 peptide.