Table 3.
Beta-lactamase gene carriage and phenotypic resistance profiles of carbapenemase-producing Acinetobacter baumannii and Pseudomonas aeruginosa isolates.
| Sample Type | Organism by K-mer Spectra |
MLST 1 | State | Carbapenemase Gene | AmpC Gene | ESBL Gene | Imipenem Interpretation (BMD) | Meropenem Interpretation (BMD) | Other Resistance Phenotypes |
mCIM/eCIM Report |
|---|---|---|---|---|---|---|---|---|---|---|
| UC | Acinetobacter baumannii | ST2 | CA | OXA-23, OXA-66 | ADC-25 | None | R | R | FEP, CTX, CAZ, TZP | carbapenemase not detected |
| BC | Acinetobacter baumannii | ST2 | CA | OXA-237, OXA-66 | ADC-25 | None | I | R | CTX, CAZ, TZP | carbapenemase not detected |
| BC | Acinetobacter baumannii | ST2 | CA | OXA-237, OXA-66 | ADC-25 | None | R | R | CTX, CAZ, TZP | carbapenemase not detected |
| BC | Acinetobacter baumannii | ST235 | NJ | OXA-71 | ADC-25 | SHV-12 | R | R | FEP, CTX, CAZ, TZP | carbapenemase not detected |
| UC | Acinetobacter baumannii | ST2 | GA | OXA-23 | ADC-25 | None | R | R | FEP, TZP | carbapenemase not detected |
| UC | Acinetobacter baumannii | ST2 | GA | OXA-23, OXA-66 | ADC-25 | None | R | R | FEP, CTX, CAZ, TZP | carbapenemase not detected |
| UC | Acinetobacter baumannii | ST2 | GA | OXA-80 | ADC-25 | None | R | R | FEP, CTX, CAZ, TZP | carbapenemase not detected |
| BC | Acinetobacter baumannii | ST2 | GA | OXA-23, OXA-66 | ADC-25 | None | R | R | FEP, CTX, CAZ, TZP | carbapenemase not detected |
| UC | Acinetobacter baumannii | ST499 | NY | OXA-23, OXA-95 | ADC-25 | None | R | R | None | carbapenemase not detected |
| UC | Acinetobacter baumannii | ST2 | NY | OXA-23, OXA-82 | ADC-25 | None | R | R | FEP, CTX, CAZ, TZP | carbapenemase not detected |
| UC | Acinetobacter baumannii | ST2 | NY | OXA-407 | ADC-25 | None | R | R | FEP, CTX, CAZ, TZP | carbapenemase not detected |
| UC | Acinetobacter baumannii | ST2 | NY | OXA-23, OXA-82 | ADC-25 | None | R | R | FEP, CTX, CAZ, TZP | carbapenemase not detected |
| BC | Acinetobacter baumannii | ST203 | NM | OXA-78 | ADC-25 | None | S | S | CTX (I) | carbapenemase not detected |
| UC | Acinetobacter baumannii | ST258 | CA | OXA-23, OXA-66 | ADC-25 | None | R | R | FEP, CTX, CAZ, TZP | carbapenemase not detected |
| BC | Acinetobacter baumannii | ST2 | TN | OXA-23, OXA-66 | ADC-25 | None | R | R | FEP, CTX, CAZ, TZP | carbapenemase not detected |
| BC | Acinetobacter baumannii | ST1525-like | TN | OXA-24, OXA-317 | ADC-25 | None | R | R | TZP | carbapenemase not detected |
| BC | Acinetobacter baumannii | ST2 | IL | OXA-24, OXA-66 | ADC-25 | None | R | R | CTX, CAZ, TZP | carbapenemase not detected |
| UC | Pseudomonas aeruginosa | ST644 | CA | IMP-62, NDM-1 | PDC-430 | PME-1 | R | R | ATM, FEP, CAZ, CZA, CT, TZP | serine carbapenemase detected |
| BC | Pseudomonas aeruginosa | ST357 | CA | NDM-1 | PDC-11 | VEB-1 | R | R | ATM, FEP, CAZ, CZA, CT, TZP | metallo beta-lactamase detected |
| BC | Pseudomonas aeruginosa | ST167 | CA | IMP-15 | PDC-445 | None | R | R | FEP, CAZ, CZA, CT, TZP | metallo beta lactamase detected |
1 Multi-locus sequencing typing was carried out using the 7-locus scheme present in CLC Genomic Workbench (CLC Type with MLST scheme 1.3). When multiple MLST schemes exist, such as for Acinetobacter baumannii and Escherichia coli, the Pasteur scheme is used. When all alleles cannot be identified, the closest sequence type is listed, followed by “-like”. N/A = no scheme available for that organism. ATM: aztreonam; FEP: cefepime; CTX: cefotaxime; CAZ: ceftazidime; CZA: ceftazidime/avibactam; CT: ceftolozane/tazobactam; ETP: ertapenem; IPM: imipenem; MEM: meropenem; MEV: meropenem/vaborbactam; TZP: piperacillin/tazobactam.