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Time course of the PRP clot retraction: Aliquot of 0.6 mL of citrate PRP was incubated with 100 U of human α-thrombin for 15, 30, 60, 90, 120, and 180 min at 37 °C and clot retraction at each time point was measured. The percentage of clot retraction increased proportionally with the incubation time and reached the maximum at 90 min incubation (one-way ANOVA, * p < 0.05 compared to peak level at 180 min, n = 19).
