Table 1.
Percentage of oocytes showing the first polar body after IVM 1.
| Experimental Group | n | Degeneration (Mean% ± SEM) |
GV (Mean% ± SEM) |
GVBD (Mean% ± SEM) |
MII (Mean% ± SEM) |
|---|---|---|---|---|---|
| CONTROL | 192 | 2.2% ± 1.3 | 12.0% ± 4.4 | 13.8% ± 4.2 | 72.0% ± 4.2 |
| H2O2 100 µM | 145 | 1.2% ± 0.7 | 15.9% ± 7.7 | 44.1% ± 2.9 * | 36.7% ± 7.8 * |
| H2O2 100 µM + (ALC 0.04 mg/mL − LC 0.08 mg/mL) | 46 | 0.0% ± 0.0 | 16.3% ± 8.7 | 26.7% ± 11.7 # | 56.9% ± 3.1 |
| H2O2 100 µM + (ALC 0.2 mg/mL − LC 0.4 mg/mL) | 53 | 2.0% ± 2.0 | 9.4% ± 1.4 | 9.4% ± 1.36 # | 73.1% ± 12.8 # |
| H2O2 100 µM + (ALC 0.8 mg/mL − LC 1.6 mg/mL) | 47 | 0.0% ± 0.0 | 12.8% ± 0.8 | 12.8% ± 0.8 # | 72.1% ± 3.9 # |
| H2O2 100 µM + PLC 0.2 mg/mL | 89 | 0.0% ± 0.0 | 18.8% ± 4.0 | 12.5% ± 12.5 # | 69.3% ± 9.3 # |
| H2O2 100 µM + PLC 1.0 mg/mL | 42 | 0.0% ± 0.0 | 4.8% ± 1.2 | 4.8% ± 4.0 # | 90.5% ± 5.2 # |
| H2O2 100 µM + PLC 2.0 mg/mL | 48 | 4.2% ± 1.2 | 1.1% ± 1.1 | 33.3% ± 3.8 | 62.5% ± 6.1 # |
| H2O2 100 µM + (PLC 0.2 mg/mL − ALC 0.2 mg/mL − LC 0.4 mg/mL) | 68 | 0.0% ± 0.0 | 10.8% ± 3.4 | 17.5% ± 6.4 # | 71.7% ± 9.7 # |
1 Statistical comparison was performed by t-test. * p < 0.05 with respect to the control; # p < 0.05 compared to H2O2.