Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Sep 12;12(9):1229. doi: 10.3390/biology12091229

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Compound AKT-1005 pre-treatment reduced HIF1A expression in 2% O₂-exposed human villous explants. (a) Immunofluorescent staining of HIF1A transcription factor expression (A–F and A+–F+) in human villous explants treated with 100 μM AKT-1005 and 100 μM MitoTEMPO for 30 min, then exposed to 24 h of 2% hypoxia. A bright green color indicates HIF1A expression. Scale bar: 200 µm. (b) Quantitation of HIF1A immunofluorescence in trophoblasts: the mean optical density of whole cell surface area was calculated in four high-power fields per sample (n = 4 per group). Mann-Whitney U test, median [IQR]. Abbreviation: MT = MitoTEMPO. Normoxia vs. Hypoxia: *: p < 0.05; Hypoxia vs. H+100 µM AKT-1005: *: p < 0.05; Hypoxia vs. H+100 µM MT: *: p < 0.05; and H+100 µM AKT-1005 vs. H+100 µM MT: *: p < 0.05.

Compound AKT-1005 pre-treatment reduced HIF1A expression in 2% O₂-exposed human villous explants. (a) Immunofluorescent staining of HIF1A transcription factor expression (A–F and A+–F+) in human villous explants treated with 100 μM AKT-1005 and 100 μM MitoTEMPO for 30 min, then exposed to 24 h of 2% hypoxia. A bright green color indicates HIF1A expression. Scale bar: 200 µm. (b) Quantitation of HIF1A immunofluorescence in trophoblasts: the mean optical density of whole cell surface area was calculated in four high-power fields per sample (n = 4 per group). Mann-Whitney U test, median [IQR]. Abbreviation: MT = MitoTEMPO. Normoxia vs. Hypoxia: *: p < 0.05; Hypoxia vs. H+100 µM AKT-1005: *: p < 0.05; Hypoxia vs. H+100 µM MT: *: p < 0.05; and H+100 µM AKT-1005 vs. H+100 µM MT: *: p < 0.05.