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. 2023 Sep 12;12(9):1229. doi: 10.3390/biology12091229

Figure 6.

Figure 6

Compound AKT-1005 antioxidant pre-treatment improved mitochondrial COX activity in 2% O2-exposed human villous explants. (a) Representative COX enzyme histochemistry (A–F and A+–F+) in different groups of villous explants treated with 100 μM AKT-1005 and 100 μM MitoTEMPO for 30 min, then exposed to 2% hypoxia for 24 h. Brown staining represents COX activity. Scale bar: 200 µm. (b) COX enzyme activity in trophoblasts was quantitated by calculating the mean optical density of the DAB products in four 40× power fields per sample (n = 5 per group). Mann-Whitney U test, median [IQR]. Abbreviation: MT = MitoTEMPO. Normoxia vs. Hypoxia: **: p < 0.01; Hypoxia vs. H+100 µM AKT-1005: **: p < 0.01; Hypoxia vs. H+100 µM MT: **: p < 0.01; and H+100 µM AKT-1005 vs. H+100 µM MT: **: p < 0.01.