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. 2023 Sep 5;10(9):1044. doi: 10.3390/bioengineering10091044

Figure 5.

Figure 5

Cytotoxicity evaluation of UV + Ca-crosslinked GA2PL gels. (A,B) AlamarBlue and LDH tests to check effects cytotoxicity of gels placed atop the cell layer. Black dotted line: 70% threshold for cytotoxicity. (n = 3–5 replicates for each experimental condition; statistics: One-way ANOVA followed by Tukey’s HSD post hoc range test between all groups was employed (****, p < 0.0001; **, p < 0.05; n.s., not significant). (C) Optical microscopy of Vero cells in the presence of UV + Ca-crosslinked GelMA/alginate gels. Red arrows indicate few suffering cells (≪20%).