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. 2023 Sep 13;14:1226832. doi: 10.3389/fimmu.2023.1226832

Figure 2.

Figure 2

Effects of inhibition of MASP-2, C1s or Factor D in the bleeding model. To investigate the effects of the different complement inhibitors and inhibitory antibodies, the signal intensity relative to the first time point was monitored for activated platelets (CD62P) and fibrinogen/fibrin. To compare the curves, the Area under the Curve (AuC) was calculated. (A–C) Inhibition of MASP-2 with SGMI-2 (164 nM) resulted in no significant difference in fluorescent intensity. (D–F) The use of inhibitory antibody against C1s (1135 nM) resulted in no significant difference in fluorescent intensity. (G–I) The use of inhibitory antibodies against Factor D (307 nM) resulted in a significant reduction of CD62P signal and statistically non-significant reduction of fibrinogen/fibrin signal. Data are shown as mean with SEM, and p-values for differences between control and inhibitor groups were determined with paired t-test (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001). ns, not significant.