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. 2023 Sep 13;14:1226832. doi: 10.3389/fimmu.2023.1226832

Figure 3.

Figure 3

Effects of inhibiting C3, C5 or all complement convertases in the bleeding model. To investigate the effects of the different complement inhibitors and inhibitory antibodies, the signal intensity relative to the first time point was monitored for activated platelets (CD62P) and fibrinogen/fibrin. To compare the curves, the Area under the Curve (AuC) was calculated. (A–C) Inhibition of C3 with Cp20 (16.3 µM) resulted in significant reduction of fibrinogen/fibrin and CD62P signal. (D–F) The use of inhibitory antibodies against C5 resulted in no significant difference in CD62P signal and significant decrease in fibrinogen/fibrin signal. (G–I) Downregulating all complement convertases with the fusion protein TriFu (2 µM) resulted in a significant decrease of fibrinogen/fibrin and CD62P signal. Data are shown as mean with SEM, and p-values for differences between control and inhibitor groups were determined with paired t-test (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001). ns, not significant.