Figure 8.
High-density autologous chondrocyte implantation (HD-ACI) process. (A) Identification of non-weight bearing regions in the knee or ankle for biopsy extraction. (B) Schematic representation of the HD-ACI approach applied to the knee, comprising two sequential surgical stages: initial biopsy collection, chondrocyte isolation, and culture in the first surgery, followed by the second surgery involving precise placement of cells onto a pre-shaped membrane tailored to fit the lesion’s dimensions and configuration, achieving a cell density of 5 million cells per cm2.