Figure 2.

Design of the experiment. Step 1—Experimental infection of B. glabrata snails with S. mansoni miracidia (BH strain). Step 2—Obtaining S. mansoni cercariae. Step 3—Percutaneous infection in mice with cercarial suspension. Step 4—Kato–Katz method for counting eggs eliminated in feces performed on the 45th day after infection, before starting the therapeutic intervention, and on the 55th day after infection, before euthanasia of the experimental groups. Step 5—Therapeutic intervention with PZQ or PLUM during five consecutive days (45th to 55th day after infection) orally by gavage. Step 6—Evaluation of the therapeutic intervention through the parasitological parameters of recovery and quantification of the parasitic load, oogram, quantification of the load of eggs deposited in the hepatic and intestinal tissues, and histopathological and histomorphometric evaluation of hepatic schistosomal granulomas.