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. Author manuscript; available in PMC: 2024 Sep 11.
Published in final edited form as: Dev Cell. 2023 Jul 11;58(17):1534–1547.e6. doi: 10.1016/j.devcel.2023.06.005

Figure 7. Spock1 induces BBB properties by modulating the brain microenvironment, altering vascular cell biology non-autonomously.

Figure 7.

(A-C) A single intracranial injection of human rSPOCK1 into the brain at 5 dpf reduces mutant leakage of DBP-EGFP at 6 dpf about 50% (B) compared to controls injected with PBS alone (A), quantified in C. (D-F) Similarly, injection of rSPOCK1 at 4 dpf reduces mutant leakage of 10 kDa Dextran at 5 dpf (E), quantified in F. (F-I) The addition of rSPOCK1 reduces gelatinase activity in the mutant brain (H) compared to control injected mutants (F), quantified in I. (J-L) Vascular expression of mcamb in the midbrain is partially restored following rSPOCK1 intracranial injections (K), quantified in L. Scale bars represent 50 μm (C, E, F) and 10 μm (K). **** p<0.0001 by 2way ANOVA compared to PBS injected control spock1hm41/hm41 mutants in C and by t test in I and L, *** p<0.001 by t test in F.