KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse Anti-Fibronectin | Abcam | ab6328 |
| Rabbit Anti-Collagen IV | Abcam | ab6586 |
| Rat Anti-PLVAP | BD Biosciences | 553849 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| FITC-Gelatin | Biovision | Cat# M1303 |
| recombinant human SPOCK1 protein | R&D Systems | Cat# 2327-PI-050 |
| NHS-activated gold nanoparticles | Cytodiagnostics | Cat# CGN5K-5–1 |
| Paraformaldehyde 20% Solution, EM Grade | VWR | Cat# 15703-S |
| Sodium Cacodylate Buffer, 0.2M | VWR | Cat# 11653 |
| Aqueous Glutaraldehyde EM Grade 50% | Electron Microscopy Sciences | Cat# 16320 |
| Dextran, Alexa Fluor 647; 10,000 MW, Anionic, Fixable | Thermo Fisher | Cat# D22914 |
| Alexa Fluor 405 NHS Ester (Succinimidyl Ester) | Thermo Fisher | Cat# A30000 |
| HCR v3.0 Amplifiers and Buffers | Molecular Instruments | https://www.molecularinstruments.com/hcr-rnafish-products |
| Agarose, low gelling temperature | Sigma | A9414 |
| EZ-Link NHS-Biotin | Thermo Fisher | 20217 |
| Dextran, Alexa Fluor 488; 10,000 MW, Anionic, Fixable | Thermo Fisher | D22910 |
| Streptavidin, Alexa Fluor 405 Conjugate | Thermo Fisher | S32351 |
| N-Phenylthiourea (PTU) | Sigma | 222909 |
| Deposited Data | ||
| bulk and single cell RNA-sequencing | We collected this data | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE230236 |
| Experimental Models: Organisms/Strains | ||
| Zebrafish: AB wildtype strain | Zebrafish International Resource Centre | ZDB-GENO-960809–1 |
| Zebrafish: spock1hm41 | This study | hm41 |
| Zebrafish: spock1hm43 | This study | hm43 |
| Zebrafish: Tg(kdrl:HRAS-mCherry)s896Tg | Chi et al, 200870 | ZDB-ALT-081212–4 |
| Zebrafish: Tg(l-fabp:DBP-EGFP)Iri500Tg | Xie et al, 201029 | ZDB-ALT-120118–1 |
| Zebrafish: TgBAC(pdgfrb:EGFP)ncv22Tg | Ando et al, 201659 | ZDB-ALT-160609–1 |
| Zebrafish:Tg(plvapb:EGFP)sj3Tg | Umans et al, 201731 | ZDB-ALT-170816–3 |
| Zebrafish: Tg(actb2:memcitrine-citrine)hm30 | Xiong et al, 201471 | ZDB-TGCONSTRCT-130114–2 |
| Zebrafish: Tg(elavl3:GCaMP6s)a13203Tg | Kim et al, 201772 | ZDB-ALT-180502–2 |
| Zebrafish: Tg(−3.1neurog1:GFP)sb2Tg | Blader et al, 200373 | ZDB-ALT-030904–2 |
| Mouse: Spock1tm1Htmn | Röll et al, 200646 | MGI:3665280 |
| Zebrafish: TgKI(Spock1-mCherry)hm44 | This study | hm44 |
| Oligonucleotides | ||
| See Table S4 | ||
| Recombinant DNA | ||
| spock1-mCherry-KI | This study | |
| Software and Algorithms | ||
| SCANPY | Wolf et al, 201881 | https://github.com/scverse/scanpy |
| Fiji | Shindelin et al, 201284 | https://imagej.net/software/fiji/ |
| Ilastik | Berg et al, 201985 | https://www.ilastik.org/ |
| SPRING | Weinreb et al, 201783 | https://kleintools.hms.harvard.edu/tools/spring.html |
| RNAmapper | Miller et al, 201333 | http://www.rnamapper.org/ |