Table 2.
Lower hinge combination panel with CH2 mutations’ characterization of binding to FcRn, FcγRI (CD64), FcγRII (CD32a), FcγRIII (CD16), and C1q.
| Biacore Kinetics | FACS Cell Binding | |||||||
|---|---|---|---|---|---|---|---|---|
| IgG1 Hinge × CH2 Mutations |
FcRn (RU) |
CD64 (RU) | CD32a (RU) | CD16 (RU) | C1q (RU) |
THP-1 (MFI) | THP-1 CD3-BSA PC (MFI) | C1q Dynal FACS (MFI) |
| N/A | 40.0 | 92.0 | 4.8 | 9.8 | 155.7 | 11,755.2 | 23,338.7 | 3505.0 |
| LALA | 44.0 | 23.5 | 0.1 | 0.6 | −13.9 | 269.6 | 5274.1 | 1983.0 |
| LALA-D265A | 44.6 | 1.0 | 0.0 | −0.3 | −9.1 | 113.1 | 119.2 | 1948.0 |
| LALA-S267K | 43.0 | 1.5 | 0.0 | −0.6 | −8.7 | 119.0 | 127.5 | 797.0 |
| LALA-H268Q | 44.0 | 22.9 | 0.4 | 0.2 | −10.7 | 202.7 | 4799.2 | 2351.0 |
| LALA-D270A | 43.6 | 9.7 | 0.1 | −0.5 | −14.4 | 131.5 | 2274.4 | 769.0 |
| LALA-L309A | 27.0 | 21.6 | 0.2 | −0.5 | 3.0 | 168.3 | 4787.3 | 2519.0 |
| LALA-L309V | 43.3 | 25.2 | 0.1 | 0.2 | −2.3 | 177.8 | 4846.6 | 2143.0 |
| LALA-K322A | 38.1 | 19.9 | 0.1 | −0.4 | −20.9 | 171.8 | 4387.4 | 875.0 |
| LALA-A327G | 42.2 | 14.3 | 0.2 | 0.1 | 0.0 | 141.6 | 7006.4 | 3146.0 |
| LALA-L328A | 40.9 | 13.6 | 0.3 | 0.0 | −1.7 | 149.1 | 6424.2 | 1492.0 |
| LALA-P329A | 45.1 | 2.2 | −0.1 | −0.5 | −3.3 | 121.7 | 168.9 | 621.0 |
| LALA-P329G | 44.5 | 2.0 | −0.1 | −0.3 | −25.1 | 122.2 | 146.2 | 676.0 |
| LALA-Y296F | 41.9 | 19.1 | 0.2 | −0.1 | −3.2 | 150.9 | 3808.6 | 2077.0 |
| LALA-A330S | 43.2 | 19.9 | 0.3 | 0.2 | −2.9 | 175.3 | 4438.2 | 1703.0 |
| LALA-P331S | 40.7 | 17.7 | 0.0 | 0.0 | −22.1 | 167.5 | 4215.4 | 724.0 |
| LALA-A330S/P331S | 37.0 | 15.8 | 0.0 | −0.1 | −5.3 | 163.8 | 3840.6 | 813.0 |
| LALE | 38.9 | 3.8 | 0.1 | 0.4 | −27.3 | 126.6 | 5298.4 | 1787.0 |
| LALE-D265A | 36.8 | 0.5 | 0.0 | −0.4 | −16.4 | 125.3 | 115.4 | 2279.0 |
| LALE-S267K | 40.1 | 0.7 | −0.1 | −0.2 | −11.2 | 121.8 | 130.3 | 911.0 |
| LALE-H268Q | 39.2 | 4.3 | 0.1 | 0.6 | 5.7 | 125.7 | 4000.1 | 1934.0 |
| LALE-D270A | 40.1 | 1.8 | 0.0 | −0.1 | −19.6 | 119.7 | 545.8 | 885.0 |
| LALE-L309A | 26.0 | 4.2 | 0.1 | 0.5 | −11.7 | 124.1 | 2758.1 | 2312.0 |
| LALE-L309V | 40.9 | 4.9 | 0.1 | 0.6 | 2.3 | 122.7 | 3543.8 | 1840.0 |
| LALE-K322A | 32.4 | 4.6 | 0.0 | 0.0 | −4.9 | 120.7 | 2323.0 | 586.0 |
| LALE-L328A | 38.8 | 3.5 | 0.4 | 0.5 | −6.6 | 130.2 | 7040.7 | 1562.0 |
| LALE-P329G | 38.1 | 0.8 | −0.1 | −0.1 | −23.3 | 120.3 | 123.7 | 660.0 |
| LALE-P329A | 37.3 | 0.8 | 0.0 | −0.3 | −6.5 | 117.5 | 123.6 | 865.0 |
| LALE-A330S | 38.6 | 3.3 | 0.1 | 0.3 | −6.2 | 125.4 | 2259.5 | 1620.0 |
| LALE-P331S | 38.6 | 3.3 | 0.2 | 0.2 | −120.6 | 128.6 | 1762.6 | 725.0 |
| LALE-A330S/P331S | 35.5 | 2.6 | −0.1 | 0.0 | −21.0 | 123.0 | 1388.0 | 750.0 |
| LALAGA-P329G | 37.1 | 0.4 | −0.1 | −0.3 | −6.2 | 115.1 | 129.0 | 664.0 |