Table 3.
Lower hinge combination panel with CH2 mutations’ biophysical developability characterization.
| IgG1 Hinge × CH2 Mutations |
PSR (Score) |
AC-SINS (Δmaxλ) | HIC RT (min) | SEC (%) | SEC Aggregate after 1 h at pH 3.5 (%) | Fc Tm (°C) |
|---|---|---|---|---|---|---|
| N/A | 0.33 | 8.1 | 8.5 | 97.6 | 0.7 | 67.5 |
| LALA | 0.37 | 9.1 | 8.4 | 93.8 | −0.2 | 66.5 |
| LALA-D265A | 0.40 | 6.9 | 8.4 | 96.1 | 9.6 | 63.0 |
| LALA-S267K | 0.39 | 14.6 | 8.4 | 94.9 | −0.4 | 67.0 |
| LALA-H268Q | 0.40 | 12.6 | 8.4 | 96.3 | −0.3 | 68.0 |
| LALA-D270A | 0.41 | 17.3 | 8.4 | 96.4 | 1.0 | 68.0 |
| LALA-L309A | 0.43 | 15.0 | 8.3 | 95.3 | 0.0 | 70.0 |
| LALA-L309V | 0.42 | 17.4 | 8.3 | 96.5 | 1.8 | 67.5 |
| LALA-K322A | 0.38 | 13.9 | 8.4 | 96.0 | 2.1 | 66.5 |
| LALA-A327G | 0.38 | 9.7 | 8.4 | 98.0 | −0.3 | 67.0 |
| LALA-L328A | 0.37 | 10.8 | 8.4 | 96.2 | 6.1 | 63.0 |
| LALA-P329A | 0.38 | 10.8 | 8.4 | 94.9 | −0.4 | 67.0 |
| LALA-P329G | 0.38 | 12.8 | 8.4 | 98.0 | 1.0 | 66.5 |
| LALA-Y296F | 0.40 | 12.6 | 8.4 | 96.1 | −0.1 | 67.5 |
| LALA-A330S | 0.37 | 11.9 | 8.4 | 96.3 | 1.0 | 67.0 |
| LALA-P331S | 0.39 | 14.7 | 8.4 | 96.3 | 3.2 | 62.5 |
| LALA-A330S/P331S | 0.37 | 13.7 | 8.4 | 92.8 | 3.1 | 64.0 |
| LALE | 0.37 | 11.2 | 8.4 | 96.6 | 0.5 | 66.5 |
| LALE-D265A | 0.38 | 9.3 | 8.4 | 96.0 | 11.3 | 62.0 |
| LALE-S267K | 0.36 | 8.0 | 8.4 | 97.8 | 3.3 | 66.5 |
| LALE-H268Q | 0.35 | 9.6 | 8.4 | 97.8 | −0.7 | 66.5 |
| LALE-D270A | 0.35 | 12.7 | 8.4 | 96.1 | 0.0 | 67.5 |
| LALE-L309A | 0.36 | 12.8 | 8.3 | 96.3 | 0.4 | 68.5 |
| LALE-L309V | 0.39 | 13.4 | 8.3 | 95.4 | 0.5 | 65.5 |
| LALE-K322A | 0.33 | 13.6 | 8.4 | 95.7 | 0.1 | 65.0 |
| LALE-L328A | 0.36 | 8.4 | 8.4 | 94.1 | 8.6 | 62.0 |
| LALE-P329G | 0.36 | 11.3 | 8.4 | 97.4 | 0.7 | 66.0 |
| LALE-P329A | 0.35 | 10.5 | 8.4 | 96.5 | 0.8 | 65.0 |
| LALE-A330S | 0.35 | 13.5 | 8.4 | 96.9 | 0.8 | 65.5 |
| LALE-P331S | 0.35 | 12.8 | 8.4 | 93.9 | 1.6 | 60.5 |
| LALE-A330S/P331S | 0.35 | 12.9 | 8.4 | 94.5 | 3.1 | 61.0 |
| LALAGA-P329G | 0.38 | 16.4 | 8.4 | 96.8 | 0.6 | 65.0 |