Figure 2.

Effects of patulin (PAT) on lipid accumulation and triglyceride (TG) content in 3T3-L1 adipocytes. The 3T3-L1 cells were seeded and induced to differentiate in the presence of PAT (1 or 5 µM) for 8 days. (A) Cells were stained with Oil Red O to observe lipid droplets. (B) Oil Red O-stained cells were extracted using isopropanol, and lipid content was quantified at 550 nm using a spectrophotometer. (C) Total TG content was measured using a TG assay kit. Data are presented as the mean ± standard deviation (SD; n ≥ 3). Different letters indicate significantly different values at p < 0.05, as determined by one-way ANOVA followed by Tukey’s post hoc test.