Figure 2.

Multi-differentiating capabilities of PDLSCs. The expression of chondrocyte master gene SOX9 (A) and osteoblast RUNX2 (C) was comparable between hPDLSCs and pPDLSCs, while for, adipocytes PPARγ was higher in pPDLSCs than in hPDLSCs, p < 0.05 (B). After culture in the chondro-differentiation medium, both hPDLSC and pPDLSCs differentiated into ACAN+ chondrocytes, forming micromasses (E–G), while they did not differentiate in basal medium (M–O). Oil red O stained adipocytes (I–K) formed in the presence of adipo-differentiation medium, but not in standard medium (H–J). Osteoblasts formed mineralized nodules (O–Q) in an osteogenic medium, but not in a standard medium (L–N). Graphs indicate the mean value of gene expression ± SEM.